Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells

Martini, Veronica, Edmans, Matthew, Gubbins, Simon, Jayaraman, Siddharth, Paudyal, Basudev, Morgan, Sophie, McNee, Adam, Morin, Théo, Rijal, Pramila, Gerner, Wilhelm, Sewell, Andrew K. ORCID: https://orcid.org/0000-0003-3194-3135, Inoue, Ryo, Bailey, Mick, Connelley, Timothy, Charleston, Bryan, Townsend, Alain, Beverley, Peter and Tchilian, Elma 2022. Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells. Mucosal Immunology 15 (3) , 428–442. 10.1038/s41385-021-00478-4

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Abstract

For the first time we have defined naïve, central memory, effector memory and differentiated effector porcine CD8 T cells and analyzed their distribution in lymphoid and respiratory tissues after influenza infection or immunization, using peptide-MHC tetramers of three influenza nucleoprotein (NP) epitopes. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM) that express CD69 and downregulate CD45RA and CCR7. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. In all tissues the frequency of NP-specific CD8 cells declines over 63 days almost to background levels but is best maintained in BAL. The kinetic of influenza specific memory CD8 T cell in this natural host species differs from that in small animal models.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Additional Information:	This article is licensed under a Creative Commons Attribution 4.0 International License
Publisher:	Springer Nature [academic journals on nature.com]
ISSN:	1933-0219
Date of First Compliant Deposit:	27 June 2022
Date of Acceptance:	8 December 2021
Last Modified:	09 May 2023 16:59
URI:	https://orca.cardiff.ac.uk/id/eprint/150758

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