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Oral progenitor cell line-derived small extracellular vesicles as a treatment for preferential wound healing outcome

Knight, Rob ORCID:, Board-Davies, Emma, Brown, Helen, Clayton, Aled ORCID:, Davis, Terence ORCID:, Karatas, Ben, Burston, James, Tabi, Zsuzsanna, Falcon-Perez, Juan M, Paisey, Stephen ORCID: and Stephens, Phil ORCID: 2022. Oral progenitor cell line-derived small extracellular vesicles as a treatment for preferential wound healing outcome. Stem Cells Translational Medicine 11 (8) , pp. 861-875. 10.1093/stcltm/szac037

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Scar formation during wound repair can be devastating for affected individuals. Our group previously documented the therapeutic potential of novel progenitor cell populations from the non-scarring buccal mucosa. These Oral Mucosa Lamina Propria-Progenitor Cells (OMLP-PCs) are multipotent, immunosuppressive, and antibacterial. Small extracellular vesicles (sEVs) may play important roles in stem cell–mediated repair in varied settings; hence, we investigated sEVs from this source for wound repair. We created an hTERT immortalized OMLP-PC line (OMLPPCL ) and confrmed retention of morphology, lineage plasticity, surface markers, and functional properties. sEVs isolated from OMLP-PCL were analyzed by nanoparticle tracking analysis, Cryo-EM and fow cytometry. Compared to bone marrow–derived mesenchymal stromal cells (BM-MSC) sEVs, OMLP-PCL sEVs were more potent at driving wound healing functions, including cell proliferation and wound repopulation and downregulated myofbroblast formation. A reduced scarring potential was further demonstrated in a preclinical in vivo model. Manipulation of OMLP-PCL sEVs may provide novel options for non-scarring wound healing in clinical settings.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Publisher: Oxford University Press
ISSN: 2157-6564
Funders: Dunhill Medical Trust
Date of First Compliant Deposit: 29 June 2022
Date of Acceptance: 28 April 2022
Last Modified: 05 Jan 2024 03:11

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