Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Proper sterol distribution is required for Candida albicans hyphal formation and virulence

McCourt, Paula, Liu, Hsing-Yin, Parker, Josie E., Gallo-Ebert, Christina, Donigan, Melissa, Bata, Adam, Giordano, Caroline, Kelly, Steven L. and Nickels, Joseph T. 2016. Proper sterol distribution is required for Candida albicans hyphal formation and virulence. G3: Genes | Genomes | Genetics 6 (11) , pp. 3455-3465. 10.1534/g3.116.033969

[thumbnail of Proper Sterol Distribution Is Required for Candida albicans Hyphal Formation and Virulence.pdf]
PDF - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview


Candida albicans is an opportunistic fungus responsible for the majority of systemic fungal infections. Multiple factors contribute to C. albicans pathogenicity. C. albicans strains lacking CaArv1 are avirulent. Arv1 has a conserved Arv1 homology domain (AHD) that has a zinc-binding domain containing two cysteine clusters. Here, we explored the role of the CaAHD and zinc-binding motif in CaArv1-dependent virulence. Overall, we found that the CaAHD was necessary but not sufficient for cells to be virulent, whereas the zinc-binding domain was essential, as Caarv1/Caarv1 cells expressing the full-length zinc-binding domain mutants, Caarv1C3S and Caarv1C28S, were avirulent. Phenotypically, we found a direct correlation between the avirulence of Caarv1/Caarv1, Caarrv1AHD, Caarv1C3S, and Caarv1C28S cells and defects in bud site selection, septa formation and localization, and hyphal formation and elongation. Importantly, all avirulent mutant strains lacked the ability to maintain proper sterol distribution. Overall, our results have established the importance of the AHD and zinc-binding domain in fungal invasion, and have correlated an avirulent phenotype with the inability to maintain proper sterol distribution.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Genetics Society of America
ISSN: 2160-1836
Date of First Compliant Deposit: 8 July 2022
Date of Acceptance: 15 August 2016
Last Modified: 19 Jun 2023 17:56

Citation Data

Cited 7 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item


Downloads per month over past year

View more statistics