Characterization of amino acid substitution W20S in MgrB involved in polymyxin resistance in Klebsiella pneumoniae

Roch, Mélanie, Martins, Willames M. B. S., Sierra, Roberto, Gales, Ana C., Andrey, Diego O. and Rather, Philip N. 2022. Characterization of amino acid substitution W20S in MgrB involved in polymyxin resistance in Klebsiella pneumoniae. Microbiology Spectrum 10 (1) , e01766-21. 10.1128/spectrum.01766-21

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Abstract

In the major human pathogen Klebsiella pneumoniae, MgrB inactivation by disruptive insertion sequence (IS) elements and mutations leading to early termination are known to play an important role in polymyxin resistance. In this study, we examined a collection of invasive blaKPC-2-producing K. pneumoniae isolates belonging to the high-risk clone sequence type 258 (ST258) displaying high rates of resistance to many antimicrobials, including polymyxins. We identified a deleterious substitution (W20S) in MgrB and confirmed by genetic complementation analysis that this variant was inactive, leading to increased polymyxin B and colistin MICs.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Additional Information:	This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International license
ISSN:	21650497
Date of First Compliant Deposit:	25 July 2022
Date of Acceptance:	18 January 2022
Last Modified:	31 May 2023 18:44
URI:	https://orca.cardiff.ac.uk/id/eprint/151460

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