Increased expression of hepcidin and toll-like receptors 8 and 10 in viral keratitis

Mohammed, Imran ORCID: https://orcid.org/0000-0002-8412-0768, Abedin, Asiya, Tsintzas, Kostas, Abedin, Syed A., Otri, Ahmad M., Hopkinson, Andrew, Mathew, Manu and Dua, Harminder S. 2011. Increased expression of hepcidin and toll-like receptors 8 and 10 in viral keratitis. Cornea 30 (8) , pp. 899-904. 10.1097/ICO.0b013e31820126e5

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Abstract

Purpose: Antimicrobial peptides (AMPs) and toll-like receptors (TLRs) form part of the “chemical barrier” of the ocular surface to microbes. Evidence suggests that pathogen recognition by TLR releases AMPs, altering AMP-TLR profiles in pathological states. This study investigated ocular surface expression of AMP-TLRs in health and disease. Methods: Complementary DNA from conjunctival and corneal impression cytology samples was used for semiquantitative and quantitative polymerase chain reactions, to determine gene expression of 6 AMPs and TLRs-1-10, in healthy subjects and patients with bacterial (n = 6), viral (n = 6), Acanthamoeba (n = 3), or dry eye (n = 7) diseases. Results: Semiquantitative polymerase chain reaction showed variable AMP expression within groups and some expression patterns between groups, increased levels of LEAP (liver-expressed antimicrobial peptide)-1/hepcidin in viral disease, LEAP-2 in dry eye, and human beta defensin 3 in bacterial disease. There was no significant variability in TLR expression. Quantitative polymerase chain reaction showed significantly higher expression of LEAP-1 (P = 0.002) and TLR-8 (P = 0.023) and TLR-10 (P = 0.014) in viral keratitis and LEAP-2 (P = 0.034) in dry eye, versus controls. Conclusions: Increased expression of LEAP-1 and TLRs 8 and 10 in viral keratitis is novel; TLR-10 has not previously had a documented ligand. LEAP-2 may have a role in dry eye. Further studies will help to improve the understanding of these diseases and yield novel therapeutic interventions.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Optometry and Vision Sciences
Publisher:	Lippincott, Williams & Wilkins
ISSN:	0277-3740
Last Modified:	11 Nov 2022 09:02
URI:	https://orca.cardiff.ac.uk/id/eprint/152695

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