Determining the potential use of biosurfactants in preventing endodontic infections

Hashim, Zahraa Amer, Maillard, Jean-Yves ORCID: https://orcid.org/0000-0002-8617-9288, Wilson, Melanie Jay ORCID: https://orcid.org/0000-0002-8834-8268 and Waddington, Rachel Jane ORCID: https://orcid.org/0000-0001-5878-1434 2022. Determining the potential use of biosurfactants in preventing endodontic infections. European Journal of Oral Sciences 130 (6) , e12900. 10.1111/eos.12900

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Abstract

Microbial biofilms play a dominant role in the failure of endodontic therapies. Bacterial adhesion is the first step in the establishment of biofilms, activating the host immune response leading to tissue damage. Biosurfactants are microbe-derived tensioactive molecules with latent anti-adhesive and anti-microbial activity. This study reports the extraction and characterization of a biosurfactant from Lactobacillus (L.) plantarum (Lp-BS) and investigates its anti-microbial and anti-adhesive properties compared to rhamnolipid, a commercially available biosurfactant. Lp-BS, extracted from L. plantarum during the growth phase, was characterized as a glycoprotein, able to reduce surface tension and emulsify non-polar liquids. Proteomic analysis of Lp-BS identified three bacterial adhesin-like proteins, suggesting roles in hindering bacterial adhesion. Lp-BS did not show significant anti-microbial activity against endodontic pathogens from the Streptococcus (Strep.) anginosus group or Enterococcus (Ent.) faecalis at 50 mg/ml. However, anti-adhesive activity on abiotic surfaces was observed against both Strep. anginosus and Strep. intermedius. Rhamnolipid exhibited strong anti-microbial activity, with minimum inhibitory concentrations of 0.097 mg/ml against Strep. anginosus, and 0.048 mg/ml against Strep. constellatus and Strep. intermedius, in addition to a marked anti-adhesive activity. These findings offer preliminary evidence for the potential application of biosurfactants as an anti-microbial and/or anti-adhesive pharmacotherapy in endodontics.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy Dentistry
Publisher:	Wiley
ISSN:	1600-0722
Date of First Compliant Deposit:	19 October 2022
Date of Acceptance:	11 October 2022
Last Modified:	21 Feb 2024 02:09
URI:	https://orca.cardiff.ac.uk/id/eprint/153500

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