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Analysis of mitochondrial dynamics in adult drosophila axons

Maddison, Daniel ORCID:, Mattedi, Francesca, Vagnoni, Alessio and Smith, Gaynor Ann ORCID: 2023. Analysis of mitochondrial dynamics in adult drosophila axons. Cold Spring Harbor Protocol 2023 (2) , 107819. 10.1101/pdb.top107819

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Neuronal survival depends on the generation of ATP from an ever-changing mitochondrial network. This requires a fine balance between the constant degradation of damaged mitochondria, biogenesis of new mitochondria, movement along microtubules, dynamic processes, and adequate functional capacity to meet firing demands. The distribution of mitochondria needs to be tightly controlled throughout the entire neuron, including its projections. Axons in particular can be enormous structures compared to the size of the cell soma, and how mitochondria are maintained in these compartments is poorly defined. Mitochondrial dysfunction in neurons is associated with aging and neurodegenerative diseases, with the axon being preferentially vulnerable to destruction. Drosophila offer a unique way to study these organelles in fully differentiated adult neurons in vivo. Here, we briefly review the regulation of neuronal mitochondria in health, aging, and disease and introduce two methodological approaches to study mitochondrial dynamics and transport in axons using the Drosophila wing system.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Publisher: Cold Spring Harbor Laboratory Press
ISSN: 1940-3402
Date of First Compliant Deposit: 8 November 2022
Date of Acceptance: 30 September 2022
Last Modified: 07 Jan 2024 21:00

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