Comparative evaluation of the effects of three hydraulic calcium silicate cements on odontoblastic differentiation of human dental pulp stem cells: an in vitro study

Assadian, Hadi, Khojasteh, Arash, Ebrahimian, Zahra, Ahmadinejad, Fereshteh, Boroojeni, Helia Sadat Haeri, Bohlouli, Mahboubeh, Nekoofar, Mohammad Hossein, Dummer, Paul MH ORCID: https://orcid.org/0000-0002-0726-7467 and Nokhbatololfoghahaei, Hanieh 2022. Comparative evaluation of the effects of three hydraulic calcium silicate cements on odontoblastic differentiation of human dental pulp stem cells: an in vitro study. Journal of Applied Oral Science 30 , e20220203. 10.1590/1678-7757-2022-0203

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Abstract

Objective The study aimed to compare the response of human dental pulp stem cells (hDPSCs) towards three hydraulic calcium silicate cements (HCSCs) by measuring cytotoxicity and expression of dentinogenic genes. Methodology Dental pulps of five impacted mandibular third molars were extirpated as a source for hDPSCs. Next to culturing, hDPSCs were subjected to fluorescence-activated cell sorting after the third passage to validate stemness of the cells. Human DPSCs were exposed to diluted supernatants of OrthoMTA (OMTA), Biodentine (BD) and Calcium-Enriched Mixture (CEM) at concentrations 10, 25, 50 and 100% at the first, third and fifth day of culture. Then, cells were exposed to 10% concentrations supernatant of HCSCs to determine DSPP and DMP1 gene expression, using a quantitative polymerase-chain reaction. Data were analyzed using one-way and three-way ANOVA, followed by Tukey post hoc statistical tests. Results Optimal cell proliferation was observed in all groups, regardless of concentration and time-point. HCSC supernatants were non-cytotoxic to hDPSCs at all three time-points, except for 100% Biodentine on day five. On day seven, OMTA group significantly upregulated the expression of DSPP and DMP1 genes. On day 14, expression of DMP1 and DSPP genes were significantly higher in BD and OMTA groups, respectively. Conclusion Biodentine significantly upregulated DMP1 gene expression over 14 days, whereas CEM was associated with only minimal expression of DSPP and DMP1 .

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Universidade de São Paulo
ISSN:	1678-7765
Date of First Compliant Deposit:	12 December 2022
Date of Acceptance:	2 September 2022
Last Modified:	03 Jun 2023 17:08
URI:	https://orca.cardiff.ac.uk/id/eprint/154814

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