Increasing sample volume for microwave-assisted rapid DNA release

Wilson-Garner, Solstice, Baillie, Les ORCID: https://orcid.org/0000-0002-8186-223X and Porch, Adrian ORCID: https://orcid.org/0000-0001-5293-8883 2023. Increasing sample volume for microwave-assisted rapid DNA release. Presented at: 2022 Asia-Pacific Microwave Conference (APMC), Yokohama, Japan, 29 November 2022 - 02 December 2022. Proceedings of 2022 Asia-Pacific Microwave Conference (APMC). IEEE, pp. 638-640. 10.23919/APMC55665.2022.10000050

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Abstract

Mycobacterium Tuberculosis (M.tb) remains one of the leading causes of death in the third world and current diagnostic methods take a long time to generate a result. A microwave mediated approach (using a TM 010 cylindrical cavity at 2.5GHz) to promote DNA release has been previously suggested as the first step (i.e. in the sample processing stage) of a rapid detection but was limited by the volume of sample it could process (up to 200µl). Here we propose a new cartridge geometry able to increase the sample volume up to 10ml, processed using the same cavity applicator. In a proof of principle study, we have used this cartridge to mediate significant DNA release in 10ml samples using Mycobacterium Smegmatis (M.smeg) and Mycobacterium Abscessus (M.Abs) cultures as a substitute for M.tb.

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Published Online
Status:	Published
Schools:	Engineering Pharmacy
Publisher:	IEEE
ISBN:	9781665451086
Last Modified:	07 Mar 2023 12:30
URI:	https://orca.cardiff.ac.uk/id/eprint/157509

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