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Increasing sample volume for microwave-assisted rapid DNA release

Wilson-Garner, Solstice, Baillie, Les ORCID: and Porch, Adrian ORCID: 2023. Increasing sample volume for microwave-assisted rapid DNA release. Presented at: 2022 Asia-Pacific Microwave Conference (APMC), Yokohama, Japan, 29 November 2022 - 02 December 2022. Proceedings of 2022 Asia-Pacific Microwave Conference (APMC). IEEE, pp. 638-640. 10.23919/APMC55665.2022.10000050

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Mycobacterium Tuberculosis (M.tb) remains one of the leading causes of death in the third world and current diagnostic methods take a long time to generate a result. A microwave mediated approach (using a TM 010 cylindrical cavity at 2.5GHz) to promote DNA release has been previously suggested as the first step (i.e. in the sample processing stage) of a rapid detection but was limited by the volume of sample it could process (up to 200µl). Here we propose a new cartridge geometry able to increase the sample volume up to 10ml, processed using the same cavity applicator. In a proof of principle study, we have used this cartridge to mediate significant DNA release in 10ml samples using Mycobacterium Smegmatis (M.smeg) and Mycobacterium Abscessus (M.Abs) cultures as a substitute for M.tb.

Item Type: Conference or Workshop Item (Paper)
Date Type: Published Online
Status: Published
Schools: Engineering
Publisher: IEEE
ISBN: 9781665451086
Last Modified: 07 Mar 2023 12:30

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