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A proviral role for CpG in cytomegalovirus infection

Iversen, Ann-Charlotte, Steinkjer, Bjorg, Nilsen, Nadra, Bohnhorst, Janne, Moen, Siv Helen, Vik, Randi, Stephens, Philip ORCID:, Thomas, David William ORCID:, Benedict, Chris A. and Espevik, Terje. 2009. A proviral role for CpG in cytomegalovirus infection. Journal of Immunology 182 (9) , pp. 5672-5681. 10.4049/jimmunol.0801268

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TLR9-dependent signaling in plasmacytoid dendritic cells is a key contributor to innate immune defense to mouse CMV infection. We aimed to study the expression and potential contribution or TLR9 signaling in human CMV (HCMV) infection of primary fibroblasts. HCMV infection strongly induced TLR9 expression in two of three fibroblast types tested. Furthermore, the TLR9 ligand CpG-B induced a strong proviral effect when added shortly after HCMV infection, enhancing virus production and cell viability. However, not all CpG classes displayed proviral activity, and this correlated with their IFN-beta-inducing ability. The proviral effect or CpG-B correlated completely with concurrent viral up-regulation of TLR9 in fibroblasts. Importantly, the timing of CpG addition was a critical parameter; in striking contrast to the proviral effect, CpG addition at the time of infection blocked viral uptake and nearly abolished HCMV production. The contrasting and time-dependent effects of CpG on HCMV infectivity reveal a complex interplay between CpG, TLR9, and HCMV infection. Additionally, the data suggest a potentially harmful role for CpG in the promotion of HCMV infection. The Journal of Immunology, 2009, 182: 5672-5681.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Systems Immunity Research Institute (SIURI)
Subjects: Q Science > QR Microbiology > QR180 Immunology
Publisher: American Association of Immunologists, Inc.
ISSN: 0022-1767
Last Modified: 18 Oct 2022 13:55

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