Cupid, a cell permeable peptide derived from amoeba, capable of delivering GFP into a diverse range of species

Fenton, Daniel, Phillips, Dylan, Maddison, Anne, H. George, Christopher, Ryves, Jonathan and D. Jones, Huw 2020. Cupid, a cell permeable peptide derived from amoeba, capable of delivering GFP into a diverse range of species. Scientific Reports 10 (1) 10.1038/s41598-020-70532-x

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Abstract

Cell permeating peptides (CPPs) are attracting great interest for use as molecular delivery vehicles for the transport of biologically active cargo across the cell membrane. The sequence of a novel CPP sequence, termed ‘Cupid’, was identified from the genome of Dictyostelium discoideum. A Cupid-Green Fluorescent Protein (Cupid-GFP) fusion protein was tested on mammalian, whole plant cells, plant leaf protoplast and fungal cell cultures and observed using confocal microscopy. GFP fluorescence builds up within the cell cytosol in 60 min, demonstrating Cupid-GFP has permeated them and folded correctly into its fluorescent form. Our combined data suggest Cupid can act as a molecular vehicle capable of delivering proteins, such as GFP, into the cytosol of a variety of cells.

Item Type:	Article
Date Type:	Publication
Schools:	Biosciences
Publisher:	Nature Research
ISSN:	2045-2322
Date of Acceptance:	29 July 2020
Last Modified:	27 Jun 2023 13:30
URI:	https://orca.cardiff.ac.uk/id/eprint/160487

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