No evidence from complementary data sources of a direct glutamatergic projection from the mouse anterior cingulate area to the hippocampal formation

Andrianova, Lilya, Yanakieva, Steliana, Margetts-Smith, Gabriella, Kohli, Shivali, Brady, Erica S., Aggleton, John P. ORCID: https://orcid.org/0000-0002-5573-1308 and Craig, Michael T. 2023. No evidence from complementary data sources of a direct glutamatergic projection from the mouse anterior cingulate area to the hippocampal formation. eLife 12 , e77364. 10.7554/elife.77364

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Abstract

The connectivity and interplay between the prefrontal cortex and hippocampus underpin various key cognitive processes, with changes in these interactions being implicated in both neurodevelopmental and neurodegenerative conditions. Understanding the precise cellular connections through which this circuit is organised is, therefore, vital for understanding these same processes. Overturning earlier findings, a recent study described a novel excitatory projection from anterior cingulate area to dorsal hippocampus. We sought to validate this unexpected finding using multiple, complementary methods: anterograde and retrograde anatomical tracing, using anterograde and retrograde adeno-associated viral vectors, monosynaptic rabies tracing, and the Fast Blue classical tracer. Additionally, an extensive data search of the Allen Projection Brain Atlas database was conducted to find the stated projection within any of the deposited anatomical studies as an independent verification of our own results. However, we failed to find any evidence of a direct, monosynaptic glutamatergic projection from mouse anterior cingulate cortex to the hippocampus proper.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Psychology
Additional Information:	License information from Publisher: LICENSE 1: URL: http://creativecommons.org/licenses/by/4.0/
Publisher:	eLife Sciences Publications
Date of First Compliant Deposit:	15 August 2023
Date of Acceptance:	3 August 2023
Last Modified:	03 Oct 2023 16:52
URI:	https://orca.cardiff.ac.uk/id/eprint/161783

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