Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture.

Tjin, Monica Suryana, Chua, Alvin Wen Choong, Moreno-Moral, Aida, Chong, Li Yen, Tang, Po Yin, Harmston, Nathan Peter, Cai, Zuhua, Tan, Bien Keem and Tryggvason, Karl 2018. Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture. Nature Communications 9 , 4432. 10.1038/s41467-018-06934-3

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Abstract

The current expansion of autologous human keratinocytes to resurface severe wound defects still relies on murine feeder layer and calf serum in the cell culture system. Through our characterization efforts of the human skin basement membrane and murine feeder layer 3T3-J2, we identified two biologically relevant recombinant laminins-LN-511 and LN-421- as potential candidates to replace the murine feeder. Herein, we report a completely xeno-free and defined culture system utilizing these laminins which enables robust expansion of adult human skin keratinocytes. We demonstrate that our laminin system is comparable to the 3T3-J2 co-culture system in terms of basal markers' profile, colony-forming efficiency and the ability to form normal stratified epidermal structure in both in vitro and in vivo models. These results show that the proposed system may not only provide safer keratinocyte use in the clinics, but also facilitate the broader use of other cultured human epithelial cells in regenerative medicine.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Nature Research
ISSN:	2041-1723
Date of Acceptance:	27 September 2018
Last Modified:	24 Aug 2023 15:15
URI:	https://orca.cardiff.ac.uk/id/eprint/161882

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