MicroRNA profiles in hippocampal granule cells and plasma of rats with pilocarpine-induced epilepsy--comparison with human epileptic samples.

Roncon, Paolo, Soukupovà, Marie, Binaschi, Anna, Falcicchia, Chiara, Zucchini, Silvia, Ferracin, Manuela, Langley, Sarah R. ORCID: https://orcid.org/0000-0003-4419-476X, Petretto, Enrico, Johnson, Michael R., Marucci, Gianluca, Michelucci, Roberto, Rubboli, Guido and Simonato, Michele 2015. MicroRNA profiles in hippocampal granule cells and plasma of rats with pilocarpine-induced epilepsy--comparison with human epileptic samples. Scientific Reports 5 , 14143. 10.1038/srep14143

Full text not available from this repository.

Abstract

The identification of biomarkers of the transformation of normal to epileptic tissue would help to stratify patients at risk of epilepsy following brain injury and inform new treatment strategies. MicroRNAs (miRNAs) are an attractive option in this direction. In this study, miRNA microarrays were performed on laser-microdissected hippocampal granule cell layer (GCL) and on plasma, at different time points in the development of pilocarpine-induced epilepsy in the rat: latency, first spontaneous seizure and chronic epileptic phase. Sixty-three miRNAs were differentially expressed in the GCL when considering all time points. Three main clusters were identified that separated the control and chronic phase groups from the latency group and from the first spontaneous seizure group. MiRNAs from rats in the chronic phase were compared to those obtained from the laser-microdissected GCL of epileptic patients, identifying several miRNAs (miR-21-5p, miR-23a-5p, miR-146a-5p and miR-181c-5p) that were up-regulated in both human and rat epileptic tissue. Analysis of plasma samples revealed different levels between control and pilocarpine-treated animals for 27 miRNAs. Two main clusters were identified that segregated controls from all other groups. Those miRNAs that are altered in plasma before the first spontaneous seizure, like miR-9a-3p, may be proposed as putative biomarkers of epileptogenesis.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Nature Research
ISSN:	2045-2322
Date of Acceptance:	12 August 2015
Last Modified:	06 Sep 2023 11:00
URI:	https://orca.cardiff.ac.uk/id/eprint/162153

Actions (repository staff only)

Edit Item