Ducker, Charles, French, Stanley, Pathak, Monika, Taylor, Harry, Sainter, Adam, Askem, William, Dreveny, Ingrid, Santana, Antônio Euzébio Goulart, Pickett, John A. ORCID: https://orcid.org/0000-0002-1008-6595 and Oldham, Neil J. 2023. Characterisation of geranylgeranyl diphosphate synthase from the sandfly Lutzomyia longipalpis. Insect Biochemistry and Molecular Biology 161 , 104001. 10.1016/j.ibmb.2023.104001 |
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Abstract
Leishmaniasis is a debilitating and often fatal neglected tropical disease. Males from sub-populations of the Leishmania-harbouring sandfly, Lutzomyia longipalpis, produce the diterpene sex and aggregation pheromone, sobralene, for which geranylgeranyl diphosphate (GGPP) is the likely isoprenoid precursor. We have identified a GGPP synthase (lzGGPPS) from L. longipalpis, which was recombinantly expressed in bacteria and purified for functional and kinetic analysis. In vitro enzymatic assays using LC-MS showed that lzGGPPS is an active enzyme, capable of converting substrates dimethylallyl diphosphate (DMAPP), (E)-geranyl diphosphate (GPP), (E,E)-farnesyl diphosphate (FPP) with co-substrate isopentenyl diphosphate (IPP) into (E,E,E)-GGPP, while (Z,E)-FPP was also accepted with low efficacy. Comparison of metal cofactors for lzGGPPS highlighted Mg2+ as most efficient, giving increased GGPP output when compared against other divalent metal ions tested. In line with previously characterised GGPPS enzymes, GGPP acted as an inhibitor of lzGGPPS activity. The molecular weight in solution of lzGGPPS was determined to be ∼221 kDa by analytical SEC, suggesting a hexameric assembly, as seen in the human enzyme, and representing the first assessment of GGPPS quaternary structure in insects.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Chemistry |
Publisher: | Elsevier |
ISSN: | 0965-1748 |
Funders: | BBSRC |
Date of First Compliant Deposit: | 22 September 2023 |
Date of Acceptance: | 20 August 2023 |
Last Modified: | 28 Sep 2023 03:52 |
URI: | https://orca.cardiff.ac.uk/id/eprint/162684 |
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