Gene gun DiOlistic labelling of retinal ganglion cells

Cimaglia, Gloria, Bevan, Ryan J. ORCID: https://orcid.org/0000-0002-2557-2887, Want, Andrew and Morgan, James E. ORCID: https://orcid.org/0000-0002-8920-1065 2023. Gene gun DiOlistic labelling of retinal ganglion cells. Mead, B., ed. Retinal Ganglion Cells: Methods and Protocols, Vol. 2708. New York, NY: Springer, n/a. (10.1007/978-1-0716-3409-7_4)

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Abstract

Gene gun DiOlistic labelling enables the detailed visualization of retinal ganglion cells (RGCs) dendritic structure. Since the level of labelling is independent of cellular health, it is useful for the characterization of neuronal structure in degenerating neurons where expressed reporters may be inadequate. The method uses compressed helium gas to fire tungsten or gold microparticles coated in carbocyanine dyes (DiD, DiI, DiO) into flat mounted retinas. Here we describe the methods to optimize labelling and ensure a high yield of adequately labelled cells, with a focus on retinal ganglion cells.

Item Type:	Book Section
Date Type:	Published Online
Status:	Published
Schools:	Optometry and Vision Sciences MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Publisher:	Springer
ISBN:	9781071634080
ISSN:	1064-3745
Date of First Compliant Deposit:	17 October 2023
Date of Acceptance:	10 August 2023
Last Modified:	31 May 2024 21:22
URI:	https://orca.cardiff.ac.uk/id/eprint/163230

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