A new modified fluorescein strip: Its repeatability and usefulness in tear film break-up time analysis.

Pult, H. ORCID: https://orcid.org/0000-0003-4371-8007 and Riede-Pult, B.H. 2012. A new modified fluorescein strip: Its repeatability and usefulness in tear film break-up time analysis. Contact Lens & Anterior Eye 35 (1) , pp. 35-38. 10.1016/j.clae.2011.07.005

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Abstract

Purpose To (i) analyze the repeatability of fluorescein instillation from a modified fluorescein strip (MFS) compared to a standard fluorescein strip (FS), and to (ii) observe its usefulness in the measurement of the fluorescein break-up time (FBUT) in comparison to the Tearscope (NIBUT). Methods In-vitro: Intra- and inter-observer repeatability in fluorescein instillation from the MFS and FS was evaluated by fluorescence analysis (n = 10, each). In-vivo: BUT of the right eye of 20 randomly selected subjects (mean age 43.3 ± 11.5, range = 21–60 years, 8 males, 12 females) was measured by use of the Tearscope and MFS. Subjects were grouped by the Ocular Surface Disease Index (OSDI) score into 8 OSDI+ and 12 OSDI− by a cut-off value of 15. Results In-vitro: Intra-observer 95% limit of agreement (LoA) of the MFS was similar to the FS LoA in observer 1 (O1), but better than the FS LoA in observer (O2) (MFS: O1: LoA = ±1.98 mW; p = 0.179; O2: ±2.71; 0.442; FS: O1: ±1.71; 0.246; O2: ±4.11; 0.512). Inter-observer LoA in fluorescence was better in MFS (±1.42; 0.111) than in FS (±3.71; 0.003). In-vivo: MFS-BUT was significantly shorter than the NIBUT (p = 0.002), but significantly correlated (r = 0.864, p < 0.001). NIBUT and MFS-BUT were significant discriminators (p < 0.001) of OSDI ± (0.948/8 s and 0.938/5 s [AUC/cut-off value]; NIBUT and MFS-BUT, respectively). Conclusions The MFS was better in the repeatability of fluorescein instillation than the FS. NIBUT and MFS-BUT were good discriminators of dry eye symptoms, but differ in their cut-off values.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Publisher:	Elsevier
ISSN:	1367-0484
Last Modified:	29 Nov 2023 15:45
URI:	https://orca.cardiff.ac.uk/id/eprint/164170

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