Carriage and within-host diversity of mcr-1.1-harboring Escherichia coli from pregnant mothers: inter- and intra-mother transmission dynamics of mcr-1.1

Naha, Sharmi, Basak, Priyanka, Sands, Kirsty, Milton, Rebecca, Carvalho, Maria J., Mitra, Shravani, Bhattacharjee, Amrita, Sinha, Anuradha, Mukherjee, Suchandra, Saha, Bijan, Chattopadhyay, Pinaki, Chakravorty, Partha Sarathi, Nandy, Ranjan Kumar, Dutta, Shanta, Walsh, Timothy R. ORCID: https://orcid.org/0000-0003-4315-4096 and Basu, Sulagna 2023. Carriage and within-host diversity of mcr-1.1-harboring Escherichia coli from pregnant mothers: inter- and intra-mother transmission dynamics of mcr-1.1. Emerging Microbes & Infections 12 , 2278899. 10.1080/22221751.2023.2278899

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Abstract

Exchange of antimicrobial resistance genes via mobile genetic elements occur in the gut which can be transferred from mother to neonate during birth. This study is the first to analyze transmissible colistin resistance gene, mcr, in pregnant mothers and neonates. Samples were collected from pregnant mothers (rectal) and septicaemic neonates (rectal & blood) and analyzed for presence of mcr, its transmissibility, genome diversity, and exchange of mcr between isolates within an individualand across different individuals (not necessarily mother-baby pairs). mcr-1.1 was detected in rectal samples of pregnant mothers (n=10, 0.9%), but not in neonates. All mcr-positive mothers gave birth to healthy neonates from whom rectal specimen were not collected. Hence, transmission of mcr between these mother-neonate pairs could not be studied. mcr-1.1 was noted only in Escherichia coli (phylogroup A & B1), and carried few resistance and virulence genes. Isolates belonged to diverse sequence types (n=11) with two novel STs (ST12452, ST12455). mcr-1.1 was borne on conjugative IncHI2 bracketed between ISApl1 on Tn6630, and the plasmids exhibited similarities in sequences across the study isolates. Phylogenetic comparison showed that study isolates were related to mcr-positive isolates of animal origin from Southeast Asian countries. Spread of mcr-1.1 within this study occurred either via similar mcr-positive clones or similar mcr-bearing plasmids in mothers. Though this study could not build evidence for mother-baby transmission, but presence of such genes in the maternal specimen may enhance the chances of transmission to neonates.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	Taylor and Francis Group
ISSN:	2222-1751
Date of First Compliant Deposit:	24 November 2023
Date of Acceptance:	6 November 2023
Last Modified:	16 Jan 2024 15:41
URI:	https://orca.cardiff.ac.uk/id/eprint/164351

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