Visual pigment concentration and photoreceptor outer segment length in the human retina

Pattni, Krishna, Wood, Ashley ORCID: https://orcid.org/0000-0002-9312-6184, Cassels, Nicola and Margrain, Tom ORCID: https://orcid.org/0000-0003-1280-0809 2024. Visual pigment concentration and photoreceptor outer segment length in the human retina. Ophthalmic and Physiological Optics 44 (5) , pp. 917-924. 10.1111/opo.13307

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Abstract

Purpose: The Beer–Lambert law suggests that visual pigment optical density (OD) should be linearly related to the length of photoreceptor outer segments (POSs). Mammalian studies indicate that visual pigment concentration increases with POS length, but the nature of this relationship may vary due to factors such as visual pigment packing density or retinal eccentricity, and may not necessarily be linearly related. The purpose of this study was to establish the relationship between OD and POS length in humans. Methods: Spectral domain optical coherence tomography (OCT) was used to image POS, and imaging retinal densitometry (IRD) was used to measure OD at corresponding locations in 19 healthy participants (age range 25–82 years). POS length and OD measurements were extracted from OCT and IRD images at 23 discrete locations spanning the central 9° of the retina. The averaged data from all participants were fitted with models based on the Beer–Lambert law to establish the relationship between OD and POS length. Results: Visual pigment OD increased monotonically with POS length, but the relationship was non‐linear, and a straight‐line fit, based on a simple interpretation of the Beer–Lambert law, provided a poor description. A model allowing for different rod and cone visual pigment concentrations provided a superior fit. Specifically, the data were well described by a model where the molar concentration of visual pigment in cones and rods were 3.8 × 10−3 mol/L and 1.8 × 10−3mol/L, respectively. Conclusions: In accordance with the Beer–Lambert law, the results indicate that OD increases monotonically with POS length in humans, but the precise relationship is dependent on photoreceptor type. These results suggest that visual pigment concentration in rods is only about 48% of that found in cones. This may be due to the ubiquitous nature of artificial light that works to reduce the concentration of rhodopsin in rod photoreceptors.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Additional Information:	License information from Publisher: LICENSE 1: URL: http://creativecommons.org/licenses/by/4.0/
Publisher:	Wiley
ISSN:	0275-5408
Date of First Compliant Deposit:	8 April 2024
Date of Acceptance:	15 March 2024
Last Modified:	04 Jul 2024 14:24
URI:	https://orca.cardiff.ac.uk/id/eprint/167783

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