Two‐dimensional liquid chromatography analysis of all‐<i>trans‐</i>, 9‐<i>cis‐</i>, and 13‐<i>cis‐</i>astaxanthin in raw extracts from <i>Phaffia rhodozyma</i>

Du, Xiping, Wang, Chun, Wu, Ling, Li, Zhipeng, Sadiq, Faizan Ahmed ORCID: https://orcid.org/0000-0003-1596-4155, Jiang, Zedong, Chen, Feng, Ni, Hui and Li, Qingbiao 2020. Two‐dimensional liquid chromatography analysis of all‐<i>trans‐</i>, 9‐<i>cis‐</i>, and 13‐<i>cis‐</i>astaxanthin in raw extracts from <i>Phaffia rhodozyma</i>. Journal of Separation Science 43 (16) , pp. 3206-3215. 10.1002/jssc.202000257

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Abstract

An effective two-dimensional liquid chromatography method has been established for the analysis of all-trans-astaxanthin and its geometric isomers from Phaffia rhodozyma employing a C18 column at the first dimension and a C30 column in the second dimension, connected by a 10-port valve using the photo-diode array detector. The regression equation of astaxanthin calibration curve was established, and the precision and accuracy values were found to be in the range of 0.32–1.14% and 98.21–106.13%, respectively. By using two-dimensional liquid chromatography, it was found that day light, ultrasonic treatment, and heat treatment have significant influence on the content of all-trans-astaxanthin in the extract from P. rhodozyma due to the transformation of all-trans-astaxanthin to cis-astaxanthin. The day light and ultrasonic treatments more likely transform all-trans-astaxanthin to 9-cis-astaxanthin, and the thermal treatment transforms all-trans-astaxanthin to 13-cis-astaxanthin. These results indicate that the two-dimensional liquid chromatography method can facilitate monitoring astaxanthin isomerization in the raw extract from P. rhodozyma. In addition, the study will provide a general reference for monitoring other medicals and bioactive chemicals with geometric isomers.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Wiley-VCH Verlag
ISSN:	1615-9314
Last Modified:	03 May 2024 08:30
URI:	https://orca.cardiff.ac.uk/id/eprint/168591

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