HPP and SGQR peptides from silkworm pupae protein hydrolysates regulated biosynthesis of cholesterol in HepG2 cell line

Sun, Suling, Wang, Wei, Wang, Nan, Zhang, Yu, Zhu, Zuoyi, Li, Xue, Wang, Junhong, Chen, Qihe, Sadiq, Faizan Ahmed ORCID: https://orcid.org/0000-0003-1596-4155, Yang, Hua, Qi, Qianhui and Zhang, Guohua 2021. HPP and SGQR peptides from silkworm pupae protein hydrolysates regulated biosynthesis of cholesterol in HepG2 cell line. Journal of Functional Foods 77 , 104328. 10.1016/j.jff.2020.104328

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Abstract

To screen several peptides that could possibly inhibit the biosynthesis of cholesterol, we selected peptides with high 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMGCR) inhibitory activity from silkworm pupae protein hydrolysates (SPPHs). SPPH was obtained by hydrolysing the proteins with neutral proteinase, and filtered to <3, 3–5 and >5 kDa fractions. Fraction 4 (SPP) with the highest HMGCR inhibitory activity was acquired from the ultrafiltrate (<3 kDa) by Sephadex G-15 filtration. In silico predictions were executed to identify peptides with HMGCR inhibitory activity. In vitro studies showed that His-Pro-Pro (HPP) and Ser-Gly-Gln-Arg (SGQR) inhibited activity of HMGCR, decreased mRNA and protein expression of HMGCR and squalene synthase, and activated the expression of low-density lipoprotein receptor. Molecular docking revealed that H-bonding interactions were responsible in HPP and SGQR for their role in improving hypercholesterolemia. Our findings suggest that HPP and SGQR have great potential in improving hypercholesterolemia.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Elsevier
ISSN:	1756-4646
Last Modified:	07 May 2024 12:15
URI:	https://orca.cardiff.ac.uk/id/eprint/168621

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