Establishment and evaluation of a method for efficient screening of Clostridium butyricum

Yi, Zhi, Sadiq, Faizan Ahmed ORCID: https://orcid.org/0000-0003-1596-4155, Wang, Hongchao, Zhao, Jianxin, Zhang, Hao, Lu, Wenwei and Chen, Wei 2020. Establishment and evaluation of a method for efficient screening of Clostridium butyricum. Folia Microbiologica 65 , pp. 917-924. 10.1007/s12223-020-00803-7

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Abstract

At present, the traditional methods for the screening of Clostridium butyricum are not sufficiently selective and efficient. Therefore, it is necessary to establish a targeted and efficient screening method for the detection of C. butyricum. Bioinformatics was used in this study to find C. butyricum specific genes, and species-specific primers were designed based on the conserved regions of the targeted genes, followed by optimization of the PCR conditions. Methodological evaluation was carried out, and the results were compared with the traditional screening method based on Trypticase Sulfite Neomycin (TSN) selective medium. A high-efficiency PCR screening method, targeting C. butyricum species-specific primers, was established. The method was confirmed to have high specificity and sensitivity towards C. butyricum cut-off CFU 103. Compared with the traditional method, the screening success rate of C. butyricum strains increased from 0.61 to 81.91%. The PCR screening method could quickly and accurately detect C. butyricum in samples and dramatically improve screening efficiency.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Springer
ISSN:	1874-9356
Last Modified:	07 May 2024 12:45
URI:	https://orca.cardiff.ac.uk/id/eprint/168627

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