An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase

Kohlmann, Alexander, Kipps, Thomas J., Rassenti, Laura Z., Downing, James R., Shurtleff, Sheila A., Mills, Ken I., Gilkes, Amanda F., Hofmann, Wolf-Karsten, Basso, Giuseppe, Dell'Orto, Marta Campo, Foà, Robin, Chiaretti, Sabina, De Vos, John, Rauhut, Sonja, Papenhausen, Peter R., Hernández, Jesus M., Lumbreras, Eva, Yeoh, Allen E., Koay, Evelyn S., Li, Rachel, Liu, Wei-min, Williams, Paul M., Wieczorek, Lothar and Haferlach, Torsten 2008. An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase. British Journal of Haematology 142 (5) , pp. 802-807. 10.1111/j.1365-2141.2008.07261.x

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Abstract

Gene expression profiling has the potential to enhance current methods for the diagnosis of haematological malignancies. Here, we present data on 204 analyses from an international standardization programme that was conducted in 11 laboratories as a prephase to the Microarray Innovations in LEukemia (MILE) study. Each laboratory prepared two cell line samples, together with three replicate leukaemia patient lysates in two distinct stages: (i) a 5-d course of protocol training, and (ii) independent proficiency testing. Unsupervised, supervised, and r2 correlation analyses demonstrated that microarray analysis can be performed with remarkably high intra-laboratory reproducibility and with comparable quality and reliability.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	Wiley
ISSN:	0007-1048
Date of First Compliant Deposit:	15 May 2024
Date of Acceptance:	17 April 2008
Last Modified:	23 May 2024 10:30
URI:	https://orca.cardiff.ac.uk/id/eprint/168935

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