Leon-Lara, Ximena, Fichtner, Alina S., Willers, Maike, Yang, Tao, Schaper, Katharina, Riemann, Lennart, Schoning, Jennifer, Harms, Anna, Almeida, Vicente, Schimrock, Anja, Janssen, Anika, Ospina-Quintero, Laura, von Kaisenberg, Constantin, Forster, Reinhold, Eberl, Matthias  ORCID: https://orcid.org/0000-0002-9390-5348, Richter, Manuela F., Pirr, Sabine, Viemann, Dorothee and Ravens, Sarina
2024.
γδ T cell profiling in a cohort of preterm infants reveals elevated frequencies of CD83+ γδ T cells in sepsis.
Journal of Experimental Medicine
221
(7)
, e20231987.
10.1084/jem.20231987
|
Abstract
Preterm infants are at high risk of developing neonatal sepsis. γδ T cells are thought to be an important set of effector cells in neonates. Here, γδ T cells were investigated in a longitudinal cohort of preterm neonates using next-generation sequencing, flow cytometry, and functional assays. During the first year of life, the Vγ9Vδ2 T cell subset showed dynamic phenotypic changes and elevated levels of fetal-derived Vγ9Vδ2 T cells were evident in infants with sepsis. Single-cell transcriptomics identified HLA-DRhiCD83+ γδ T cells in neonatal sepsis, which expressed genes related to antigen presentation. In vitro assays showed that CD83 was expressed on activated Vγ9Vδ2 T cells in preterm and term neonates, but not in adults. In contrast, activation of adult Vγ9Vδ2 T cells enhanced CD86 expression, which was presumably the key receptor to induce CD4 T cell proliferation. Together, we provide a map of the maturation of γδ T cells after preterm birth and highlight their phenotypic diversity in infections.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Medicine |
| Publisher: | Rockefeller University Press |
| ISSN: | 0022-1007 |
| Date of Acceptance: | 22 April 2024 |
| Last Modified: | 17 May 2024 10:15 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/168968 |
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