Precisely patterned nanofibres made from extendable protein multiplexes

Bethel, Neville, Borst, Andrew, Parmeggiani, Fabio ORCID: https://orcid.org/0000-0001-8548-1090, Bick, Matthew, Brunette, TJ, Nguyen, Hannah, Kang, Alex, Bera, Asim, Carter, Lauren, Miranda, Marcos, Kibler, Ryan, Lamb, Mila, Li, Xinting, Banumathi, Sankaran and Baker, David 2023. Precisely patterned nanofibres made from extendable protein multiplexes. Nature Chemistry 15 (Decemb) , pp. 1664-1671. 10.1038/s41557-023-01314-x

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Abstract

Molecular systems with coincident cyclic and superhelical symmetry axes have considerable advantages for materials design as they can be readily lengthened or shortened by changing the length of the constituent monomers. Among proteins, alpha-helical coiled coils have such symmetric, extendable architectures, but are limited by the relatively fixed geometry and flexibility of the helical protomers. Here we describe a systematic approach to generating modular and rigid repeat protein oligomers with coincident C2 to C8 and superhelical symmetry axes that can be readily extended by repeat propagation. From these building blocks, we demonstrate that a wide range of unbounded fibres can be systematically designed by introducing hydrophilic surface patches that force staggering of the monomers; the geometry of such fibres can be precisely tuned by varying the number of repeat units in the monomer and the placement of the hydrophilic patches.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
ISSN:	1664–1671
Date of Acceptance:	4 August 2023
Last Modified:	14 Oct 2024 13:30
URI:	https://orca.cardiff.ac.uk/id/eprint/172208

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