Preparation and antidiabetic effect of orally administered Nifedipine-loaded solid lipid nanoparticles in fructose-induced diabetic rats

Sarker, Sabarni, Ali, Md. Ashraf, Barman, Ranjan Kumar, Noguchi, Shuji, Iwao, Yasunori, Itai, Shigeru and Wahed, Mir Imam Ibne 2018. Preparation and antidiabetic effect of orally administered Nifedipine-loaded solid lipid nanoparticles in fructose-induced diabetic rats. Pharmacology & Pharmacy 09 (10) , 457. 10.4236/pp.2018.910035

Preview

PDF - Published Version Available under License Creative Commons Attribution. Download (646kB) | Preview

Abstract

The use of Nifedipine (NI), a dihydropyridine calcium channel blocker, is limited due to its poor aqueous solubility. However, NI loaded solid-lipid nanoparticles (NI-SLN) are known to exhibit suitable pharmacokinetic properties and good biocompatibility. The present investigation was designed to evaluate the effects of NI-SLN on glucose homeostasis, lipid metabolism and liver function in fructose-induced diabetic rats. NI-SLN was prepared by high pressure homogenization technique followed by lyophilization with trehalose as cryoprotectant. Diabetes was induced into rats by the administration of fructose (10%) in drinking water for six weeks. After induction of diabetes, rats were divided into four groups for the oral ingestion of NI, NI-SLN and/or vehicles and their effects on blood glucose levels, oral glucose tolerance test (OGTT), lipid profile, biochemical parameters, electrolytes and histopathology were observed. Single dose administration and treatment with NI-SLN showed significant glucose lowering efficacy in fructose-induced diabetic rats. Although NI and NI-SLN did not alter the fasting blood glucose level in normal rats, diabetic rats treated with NI-SLN resulted in significant reduction in glucose level for 24 hr. In OGTT, NI-SLN exhibited significant antihyperglycemic activity in both normal and diabetic rats. So, NI-SLN has better glucose lowering efficacy than that of pure NI in diabetic rats. The survival rates in rats among the treatment groups were 100%. Treatment with NI-SLN significantly improved lipid profiles than NI alone and the effect was dose-dependent. Administration of NI-SLN significantly reduced uric acid, creatinine levels and maintained a good cationic balance. After two weeks of NI-SLN treatment, hepatocytes regained their normal architecture, and the beneficial effect could be correlated with the reduction of SGOT and total bilirubin levels. Therefore, NI-SLN was found to be useful for the enhancement of bioavailability and exhibited profound antidiabetic activity in rats. The results of the study suggested that NI-SLN exerted better improvement in glucose levels, lipid profiles and organ protection than pure NI and might have some beneficial effects in the management of diabetic patients.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Pharmacy
Publisher:	Scientific Research
ISSN:	2157-9423
Date of First Compliant Deposit:	1 October 2024
Date of Acceptance:	17 October 2018
Last Modified:	22 Oct 2024 12:52
URI:	https://orca.cardiff.ac.uk/id/eprint/172502

Actions (repository staff only)

Edit Item