Bi-directional cell-pericellular matrix interactions direct stem cell fate

Ferreira, Silvia A., Motwani, Meghna S., Faull, Peter A., Seymour, Alexis J., Yu, Tracy T. L., Enayati, Marjan, Taheem, Dheraj K., Salzlechner, Christoph, Haghighi, Tabasom, Kania, Ewa M., Oommen, Oommen P. ORCID: https://orcid.org/0000-0003-2768-0133, Ahmed, Tarek, Loaiza, Sandra, Parzych, Katarzyna, Dazzi, Francesco, Varghese, Oommen P., Festy, Frederic, Grigoriadis, Agamemnon E., Auner, Holger W., Snijders, Ambrosius P., Bozec, Laurent and Gentleman, Eileen 2018. Bi-directional cell-pericellular matrix interactions direct stem cell fate. Nature Communications 9 , 4049. 10.1038/s41467-018-06183-4

Full text not available from this repository.

Abstract

Modifiable hydrogels have revealed tremendous insight into how physical characteristics of cells’ 3D environment drive stem cell lineage specification. However, in native tissues, cells do not passively receive signals from their niche. Instead they actively probe and modify their pericellular space to suit their needs, yet the dynamics of cells’ reciprocal interactions with their pericellular environment when encapsulated within hydrogels remains relatively unexplored. Here, we show that human bone marrow stromal cells (hMSC) encapsulated within hyaluronic acid-based hydrogels modify their surroundings by synthesizing, secreting and arranging proteins pericellularly or by degrading the hydrogel. hMSC’s interactions with this local environment have a role in regulating hMSC fate, with a secreted proteinaceous pericellular matrix associated with adipogenesis, and degradation with osteogenesis. Our observations suggest that hMSC participate in a bi-directional interplay between the properties of their 3D milieu and their own secreted pericellular matrix, and that this combination of interactions drives fate.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Publisher:	Nature Research
ISSN:	2041-1723
Last Modified:	22 Oct 2024 17:30
URI:	https://orca.cardiff.ac.uk/id/eprint/172989

Actions (repository staff only)

Edit Item