Saline accelerates oxime reaction with aldehyde and keto substrates at physiological pH

Wang, Shujiang, Nawale, Ganesh N., Kadekar, Sandeep, Oommen, Oommen P. ORCID: https://orcid.org/0000-0003-2768-0133, Jena, Naresh K., Sudip, Chakraborty, Hilborn, Jöns and Varghese, Oommen P. 2018. Saline accelerates oxime reaction with aldehyde and keto substrates at physiological pH. Scientific Reports 8 (1) , 2193. 10.1038/s41598-018-20735-0

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Abstract

We have discovered a simple and versatile reaction condition for oxime mediated bioconjugation reaction that could be adapted for both aldehyde and keto substrates. We found that saline accelerated the oxime kinetics in a concentration-dependent manner under physiological conditions. The reaction mechanism is validated by computational studies, and the versatility of the reaction is demonstrated by cell-surface labeling experiments. Saline offers an efficient and non-toxic catalytic option for performing the bioorthogonal-coupling reaction of biomolecules at the physiological pH. This saline mediated bioconjugation reaction represents the most biofriendly, mild and versatile approach for conjugating sensitive biomolecules and does not require any extensive purification step.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Publisher:	Nature Research
ISSN:	2045-2322
Last Modified:	22 Oct 2024 17:00
URI:	https://orca.cardiff.ac.uk/id/eprint/173020

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