Development of a method for fast assessment of protein solubility based on ultrasonic dispersion and differential centrifugation technology

Wei, Dongwei, Wang, Meng, Wang, Hongdi, Liu, Guijin, Fang, Jun and Jiang, Yanbin 2022. Development of a method for fast assessment of protein solubility based on ultrasonic dispersion and differential centrifugation technology. ACS Omega 7 (35) , 31338–31347. 10.1021/acsomega.2c03666

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Abstract

Protein solubility is very important for protein crystallization, bioprocess development, and protein application. In this study, a method based on the stability of a protein dispersion system is proposed for fast assessment of protein solubility, which mainly involves ultrasonic dispersion, differential centrifugation, and spectral measurement (UDDCS) and curvature estimation. The appropriate ultrasonic time and centrifugal time were experimentally determined at first. The results show that the relationship between the standard deviation and the protein concentrations originally added accords with the modified exponential equation, and the corresponding concentration of the maximum curvature point is defined as the solubility of the protein. Lysozyme solubility data in NaCl aqueous solutions and zein solubility data in ethanol aqueous solutions are selected to verify the UDDCS method by comparing the data obtained by the UDDCS method and the results from references, and the results indicate that the UDDCS method is reliable, universal, and time-saving. Finally, measurements of zein solubility in NaOH solution and casein solubility in urea aqueous solution were conducted as test cases by the UDDCS method.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Engineering
Publisher:	American Chemical Society
ISSN:	2470-1343
Date of Acceptance:	10 August 2022
Last Modified:	28 Feb 2025 15:00
URI:	https://orca.cardiff.ac.uk/id/eprint/176441

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