Gold nanoparticle incorporated graphene oxide as a SERS platform for ultratrace antibody free sensing of the cancer biomarker CEA

Basu, Shalmali, Rana, Nabakumar, Morgan, David ORCID: https://orcid.org/0000-0002-6571-5731 and Sen, Kamalika 2025. Gold nanoparticle incorporated graphene oxide as a SERS platform for ultratrace antibody free sensing of the cancer biomarker CEA. Langmuir 41 (11) , 7886–7901. 10.1021/acs.langmuir.5c00522

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Abstract

A simple, fast, low-cost, and efficient method is designed for the synthesis of graphene oxide (GO) (20 nm) from graphite using a strong oxidant Ce(IV). GO is further modified with gold nanoparticles (AuNPs) (5-8 nm) to generate a AuGO nanocomposite (25 nm). Raman spectral analyses confirm that the synthesized AuGO has a potential selective sensing ability for the cancer biomarker carcinoembryonic antigen (CEA) in serum. Sensing assays are also carried out in the presence of high concentrations of glucose, cholesterol, and insulin using this method, which become significantly elevated in conditions of different pathophysiological disorders. Ultratrace antibody free sensing of CEA in serum is achieved using surface-enhanced Raman spectroscopy with an amazing LOD of 12.5 fg/mL. The interaction between CEA and AuGO is further established using Raman, fluorescence, circular dichroism spectroscopy, and theoretical studies. The specificity of sensing is tested by checking the response in the presence of other cancer biomarkers, such as CA 19-9, CA 125, and PSA, which do not show any signal enhancement with AuGO in Raman spectroscopy.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Chemistry Research Institutes & Centres > Cardiff Catalysis Institute (CCI)
Publisher:	American Chemical Society
ISSN:	0743-7463
Date of First Compliant Deposit:	2 April 2025
Date of Acceptance:	27 February 2025
Last Modified:	10 Mar 2026 02:45
URI:	https://orca.cardiff.ac.uk/id/eprint/177171

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