Identifying Alzheimer’s disease genes in apolipoprotein E−/− mice brains with confirmed Porphyromonas gingivalis entry

Singhrao, Sim K. and Consoli, Claudia 2025. Identifying Alzheimer’s disease genes in apolipoprotein E−/− mice brains with confirmed Porphyromonas gingivalis entry. Journal of Alzheimer's Disease Reports 9 10.1177/25424823251332874

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Abstract

BackgroundThe apolipoprotein E allele ε4 is the most well-known predisposing genetic risk factor for Alzheimer's disease (AD).ObjectiveTo identify AD genes in apolipoprotein E-/- (ApoE-/-) mice brains with confirmed entry of Porphyromonas gingivalis.MethodsTaqMan™ Mouse AD arrays were performed on orally infected ApoE-/- mice with confirmed P. gingivalis entry and compared with sham infected mice brains (N = 4) at 12- and 24-weeks post infection.ResultsGene expression by qPCR demonstrated that in the P. gingivalis 12-weeks post oral infection, two genes were statistically significantly changed in their expression. These were cyclin dependent kinase 5 regulatory subunit 1 (Cdk5r1, 0.15 logfold change, p = 0.05) and Interleukin 1 alpha, (IL1a, -0.10 log fold change, p = 0.012). In the P. gingivalis 24-weeks post oral infection, three genes were statistically significantly changed in their expression. These were cholinergic receptor nicotinic alpha 7 subunit or Chrna7 (0.10 log fold change, p = 0.02), mitogen-activated protein kinase 1 or Mapk1 (0.10 log fold change, p = 0.05) and visinin like 1 or Vnsl1 (0.01 log fold change, p = 0.04). 87 out of 92 AD target genes demonstrated no difference between infected and sham mice brains.ConclusionsFive genes, from a recognized AD panel had statistically significantly altered expression in the ApoE-/- mouse AD model following P. gingivalis entry into the brain. This suggests the ApoE-/- genetic variation may control the biological activity of specific genes relevant to inflammation and neuronal plasticity following P. gingivalis infection.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Schools > Medicine
Additional Information:	License information from Publisher: LICENSE 1: Title: cc by, Type: cc by
Publisher:	SAGE Publications
ISSN:	2542-4823
Date of First Compliant Deposit:	10 April 2025
Last Modified:	10 Apr 2025 11:45
URI:	https://orca.cardiff.ac.uk/id/eprint/177576

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