The impact of passive ultrasonic irrigation on the bond strength of two different self-etch adhesives to human pulp chamber dentine: a laboratory investigation

Turky, Mohammed, Matinlinna, Jukka, Lukomska-Szymanska, Monika, Nagendrababu, Venkateshbabu, Dummer, Paul M. H. ORCID: https://orcid.org/0000-0002-0726-7467, Elheeny, Ahmad Abdel Hamid and Mahmoud, Nermin Alsayed 2025. The impact of passive ultrasonic irrigation on the bond strength of two different self-etch adhesives to human pulp chamber dentine: a laboratory investigation. BMC Oral Health 25 (1) , 550. 10.1186/s12903-025-05858-x

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Abstract

Objectives: To evaluate the impact of passive ultrasonic irrigation on the micro-tensile bond strength of two different self-etch adhesive systems, including a universal one-step adhesive and a two-step adhesive system, to pulp chamber dentine compared to conventional syringe irrigation. Methods: Twenty-four extracted human mandibular first molar teeth were chosen according to strict criteria and mounted in epoxy resin blocks. Subsequently, the pulp chambers were exposed using an Isomet cutting machine. The specimens were numbered and assigned to four groups (6 teeth each) based on the canal irrigation method and the adhesive system used as follows: Conventional syringe irrigation in which a universal one-step self-etch adhesive system was applied (CSIU), conventional syringe irrigation in which a two-step self-etch adhesive system was used (CSIT), passive ultrasonic irrigation in which a universal one-step self-etch adhesive system was utilized (PUIU), and passive ultrasonic irrigation in which a two-step self-etch adhesive system was employed (PUIT). Following placement of the final restoration and submission to simultaneous thermo-mechanical cycling (artificial aging) equivalent to 6-month intra-oral aging, the teeth were sectioned and dentine/restoration beams were prepared. The micro-tensile bond strength was evaluated and the failure mode was defined, with a confirmatory evaluation of the dentine-resin interface using a scanning electron microscope. Statistical analysis was conducted using one-way ANOVA and Tukey's post hoc tests to compare irrigation regimens for each adhesive technique independently, while failure modes of each adhesive system were represented as the frequency for each irrigation method. The significance level was set at 5%, with a confidence interval (CI) of 95%.ResultsThe micro-tensile bond strength of composite resin restorations to pulp chamber dentine was reduced significantly with ultrasonic irrigation with more unfavorable failure modes compared to syringe irrigation (P Conclusions: Within the limitations of the present laboratory investigation, ultrasonic irrigation may negatively affect the bond between composite resin restorations and pulp chamber dentine compared to conventional syringe irrigation. The two-step self-etch adhesive tended to achieve a stronger bond to pulp chamber dentine than the universal one-step self-etch adhesive. Clinical relevance: While ultrasonic irrigation would be essential for effective root canal debridement and disinfection, it is imperative for clinicians to consider its potential adverse effects. This method may considerably impact the bond strength of composite resin restorations to the pulp chamber dentine, particularly when compared to conventional syringe irrigation. In root canal-treated teeth, a two-step self-etch adhesive system might be more effective in maximizing the bond strength to pulp chamber dentine than a universal adhesive system. However, these findings were concluded under the conditions of the present study and must be interpreted cautiously. Further research is recommended to validate these results and fully understand the clinical ramifications of ultrasonic irrigation on adhesive performance in different dental situations. Clinical trial number: Non-applicable. Conducting the current experiment was limited to the approval of the local Research Ethics Committee at the Faculty of Dentistry, Minia University, Egypt (Committee No. 106, Registration No. 910, Date: April 30, 2024).

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Schools > Dentistry
Additional Information:	License information from Publisher: LICENSE 1: Title: cc by, Type: cc by
Publisher:	BioMed Central
ISSN:	1472-6831
Date of First Compliant Deposit:	29 April 2025
Last Modified:	29 Apr 2025 10:45
URI:	https://orca.cardiff.ac.uk/id/eprint/177962

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