A longitudinal study assessing Aspergillus PCR performance through participation of external quality assessment schemes from 2019 to 2022

Ricketts, Alastair, Montgomery, Dorothy, Loeffler, Juergen, Barnes, Rosemary, Cruciani, Mario, Donnelly, J. Peter and White, P. Lewis 2025. A longitudinal study assessing Aspergillus PCR performance through participation of external quality assessment schemes from 2019 to 2022. Medical Mycology 63 (5) , myaf036. 10.1093/mmy/myaf036

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Abstract

Aspergillus PCR now employs standardised methodology and commercial options, with clinical performance validated through meta-analyses. The long-term availability of external quality assessment schemes drives performance and provides a currently untapped source of technical data. The Fungal PCR initiative and the Quality Control for Molecular diagnostics (QCMD) combined forces to evaluate data from the Aspergillus PCR QCMD schemes between 2019-2022. Analysis showed inferior detection of non-fumigatus Aspergillus species (i.e., A. niger) compared with A. fumigatus. Longitudinal evaluation showed the use of “in-house” PCR methods by new participants when testing plasma generated lower positivity rates compared to commercial assays.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Medicine
Publisher:	Oxford University Press
ISSN:	1369-3786
Date of First Compliant Deposit:	21 May 2025
Date of Acceptance:	10 April 2025
Last Modified:	11 Apr 2026 01:45
URI:	https://orca.cardiff.ac.uk/id/eprint/177977

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