Bidirectional control of generalized absence epilepsy networks via real-time direct depolarization of thalamocortical neurons

Morais, Tatiana P., Taylor, Hannah L., Nagy, Olivér, Mátyás, Ferenc, David, Francois, Crunelli, Vincenzo and Lőrincz, Magor L. 2025. Bidirectional control of generalized absence epilepsy networks via real-time direct depolarization of thalamocortical neurons. Progress in Neurobiology 252 , 102774. 10.1016/j.pneurobio.2025.102774

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Abstract

Absence seizures (ASs), characterized by bilateral spike-and-wave discharges (SWDs), are a hallmark of idiopathic generalized epilepsies. We investigated the role of thalamocortical (TC) neurons in the generation and termination of ASs using optogenetic techniques in freely behaving GAERS rats, a well-established AS model. We demonstrate that direct depolarization of ChR2-transfected TC neurons in the ventrobasal thalamic nuclei during quiet wakefulness (QW) reliably elicits ethosuximide-sensitive ASs that have similar duration and frequency to those of spontaneous ASs, while showing little and no effect during active wakefulness (AW) and slow wave sleep (SWS), respectively. Light-stimulation of TC neurons fails to elicit ASs during AW, QW and SWS in non-epileptic control (NEC) rats, whereas it could evoke short ASs in Wistar rats, prevalently during QW. Notably, brief light stimulation effectively halted ongoing spontaneous ASs in GAERS rats (i.e. both SWDs and immobility), immediately altering thalamic multi-unit activity from rhythmic to irregular firing, irrespective of the SWD phase at which it was delivered. These findings support the view that the excitability of cortico-thalamic-cortical network is highly behavioural state-dependent, with increased susceptibility to the induction of ASs during QW, thus questioning the necessity of low-threshold burst firing of TC neurons in the generation of these seizures. Moreover, they highlight the dual control of ASs by TC neurons, underscoring their potential as therapeutic targets for AS modulation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Biosciences
Publisher:	Elsevier
ISSN:	0301-0082
Date of First Compliant Deposit:	2 June 2025
Date of Acceptance:	29 April 2025
Last Modified:	03 Sep 2025 12:30
URI:	https://orca.cardiff.ac.uk/id/eprint/178670

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