Improving molecular epidemiological surveillance of strongyloidiasis upon differentiation of strongyloides fuelleborni fuelleborni from strongyloides stercoralis

Cunningham, Lucas J, Nevin, William D, Verweij, Jaco J, Buonfrate, Dora, Scarso, Salvatore, Khieu, Virak, O’Ferrall, Angus M, Rollason, Sarah and Stothard, J Russell 2025. Improving molecular epidemiological surveillance of strongyloidiasis upon differentiation of strongyloides fuelleborni fuelleborni from strongyloides stercoralis. The Journal of Infectious Diseases 10.1093/infdis/jiaf237

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Abstract

Molecular epidemiological surveillance for zoonotic strongyloidiasis is confounded by a genus-specific TaqMan probe assay that conflates Strongyloides fuelleborni fuelleborni with Strongyloides stercoralis. To improve surveillance, we developed and validated a novel duplex species-specific TaqMan probe assay, screening a representative collection of available clinical samples. Our assay was highly discriminatory, evidencing no cross-reactivity, and had a lowest DNA detection limit of 1 pg/µL. However, as the genus-specific DNA assay has greater detection ability, we propose a 2-step protocol where samples are first screened with this assay then, if positive, screened with our species-specific assay, discriminating (co)infections between each threadworm species.

Item Type:	Article
Date Type:	Published Online
Status:	In Press
Schools:	Schools > Biosciences
Additional Information:	License information from Publisher: LICENSE 1: URL: https://creativecommons.org/licenses/by-nc-nd/4.0/, Start Date: 2025-05-27
Publisher:	Oxford University Press
ISSN:	0022-1899
Date of First Compliant Deposit:	11 June 2025
Date of Acceptance:	6 May 2025
Last Modified:	11 Jun 2025 12:00
URI:	https://orca.cardiff.ac.uk/id/eprint/179003

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