Cunningham, Lucas J, Nevin, William D, Verweij, Jaco J, Buonfrate, Dora, Scarso, Salvatore, Khieu, Virak, O’Ferrall, Angus M, Rollason, Sarah and Stothard, J Russell
2025.
Improving molecular epidemiological surveillance of strongyloidiasis upon differentiation of strongyloides fuelleborni fuelleborni from strongyloides stercoralis.
The Journal of Infectious Diseases
10.1093/infdis/jiaf237
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Abstract
Molecular epidemiological surveillance for zoonotic strongyloidiasis is confounded by a genus-specific TaqMan probe assay that conflates Strongyloides fuelleborni fuelleborni with Strongyloides stercoralis. To improve surveillance, we developed and validated a novel duplex species-specific TaqMan probe assay, screening a representative collection of available clinical samples. Our assay was highly discriminatory, evidencing no cross-reactivity, and had a lowest DNA detection limit of 1 pg/µL. However, as the genus-specific DNA assay has greater detection ability, we propose a 2-step protocol where samples are first screened with this assay then, if positive, screened with our species-specific assay, discriminating (co)infections between each threadworm species.
Item Type: | Article |
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Date Type: | Published Online |
Status: | In Press |
Schools: | Schools > Biosciences |
Additional Information: | License information from Publisher: LICENSE 1: URL: https://creativecommons.org/licenses/by-nc-nd/4.0/, Start Date: 2025-05-27 |
Publisher: | Oxford University Press |
ISSN: | 0022-1899 |
Date of First Compliant Deposit: | 11 June 2025 |
Date of Acceptance: | 6 May 2025 |
Last Modified: | 11 Jun 2025 12:00 |
URI: | https://orca.cardiff.ac.uk/id/eprint/179003 |
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