Cunningham, Lucas J., Nevin, William D., Verweij, Jaco J., Buonfrate, Dora, Scarso, Salvatore, Khieu, Virak, O’Ferrall, Angus M., Rollason, Sarah and Stothard, J. Russell
2025.
Improving molecular epidemiological surveillance of strongyloidiasis upon differentiation of strongyloides fuelleborni fuelleborni from strongyloides stercoralis.
The Journal of Infectious Diseases
232
(1)
, e169-e173.
10.1093/infdis/jiaf237
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Abstract
Molecular epidemiological surveillance for zoonotic strongyloidiasis is confounded by a genus-specific TaqMan probe assay that conflates Strongyloides fuelleborni fuelleborni with Strongyloides stercoralis. To improve surveillance, we developed and validated a novel duplex species-specific TaqMan probe assay, screening a representative collection of available clinical samples. Our assay was highly discriminatory, evidencing no cross-reactivity, and had a lowest DNA detection limit of 1 pg/µL. However, as the genus-specific DNA assay has greater detection ability, we propose a 2-step protocol where samples are first screened with this assay then, if positive, screened with our species-specific assay, discriminating (co)infections between each threadworm species.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Schools > Biosciences |
Additional Information: | License information from Publisher: LICENSE 1: URL: https://creativecommons.org/licenses/by-nc-nd/4.0/, Start Date: 2025-05-27 |
Publisher: | Oxford University Press |
ISSN: | 0022-1899 |
Date of First Compliant Deposit: | 11 June 2025 |
Date of Acceptance: | 6 May 2025 |
Last Modified: | 14 Aug 2025 09:56 |
URI: | https://orca.cardiff.ac.uk/id/eprint/179003 |
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