Killian, Christina, Macori, Guerrino, Centeleghe, Isabella, Laas, Peeter, Lewis, Rebecca, van Leuven, Nicole, Wall, Katie, Abbara, Razan, Lucassen, Ralf, Hagström, Marja, Bockmuehl, Dirk, Fielder, Mark, Perkola, Noora, Kisand, Veljo, Maillard, Jean-Yves  ORCID: https://orcid.org/0000-0002-8617-9288 and Fanning, Séamus
2025.
Deciphering the microbiota compositions of complex biofilms from hospital and domestic settings across Estonia, Germany and the United Kingdom.
FEMS Microbiology Letters
372
, fnaf118.
10.1093/femsle/fnaf118
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Abstract
Currently, there is a limited understanding of the microbiota composition of complex biofilms, in particular describing the abundance of bacterial genera, fungi and yeasts with reference to the One Health axes. As a starting point, describing the microbiota found in these settings would begin to describe the nature of any biological hazards present and facilitate development of strategies to limit transmission and the spread of infection. Furthermore, using this approach suitable interventional measures could then be tested in the laboratory scale model for their efficacy and then applied in situ. COMplex Biofilms and AMR Transmission (COMBAT) is a consortium of research teams that studied the application of next-generation sequencing (NGS) strategies to identify bacterial, fungal and yeast species present in selected biofilms recovered from drain settings found in domestic and hospital settings in four geographical regions. Findings from this study extended our understanding of the bacterial, fungal and yeast abundances in these sample biofilms and how they may change following enrichment. A VSEARCH-based high-resolution clustering approach was implemented to full-length 16S rRNA (FL-16S) sequencing reads to generate near-ASV operational units, enabling detailed characterisation of the microbial communities within complex biofilms. This analytical framework provides the basis for testing interventional measures at the laboratory scale that could be implemented to reduce risk to the One Health axes.
| Item Type: | Article |
|---|---|
| Date Type: | Published Online |
| Status: | In Press |
| Schools: | Schools > Pharmacy |
| Additional Information: | License information from Publisher: LICENSE 1: URL: https://creativecommons.org/licenses/by/4.0/, Start Date: 2025-10-23 |
| Publisher: | Oxford University Press |
| ISSN: | 0378-1097 |
| Date of First Compliant Deposit: | 8 December 2025 |
| Date of Acceptance: | 20 October 2025 |
| Last Modified: | 19 Dec 2025 10:26 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/182094 |
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