A novel method for the induction of experimental glaucoma using magnetic microspheres

Samsel, Paulina A., Kisiswa, Lilian ORCID: https://orcid.org/0000-0002-1800-0645, Erichsen, Jonathan Thor ORCID: https://orcid.org/0000-0003-1545-9853, Cross, Stephen Daniel and Morgan, James Edwards ORCID: https://orcid.org/0000-0002-8920-1065 2011. A novel method for the induction of experimental glaucoma using magnetic microspheres. Investigative Ophthalmology & Visual Science 52 (3) , pp. 1671-1675. 10.1167/iovs.09-3921

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Abstract

Purpose. The development of a method for the sustained elevation of intraocular pressure in experimental glaucoma based on the anterior chamber injection of paramagnetic microbeads. Methods. Unilateral glaucoma was induced in adult male Norwegian Brown rats by the injection of paramagnetic polystyrene microspheres. A handheld 0.45 Tesla magnet was used to draw the beads into the iridocorneal angle to impede aqueous drainage via the trabecular meshwork. Results. Elevated intraocular pressures (IOPs) were induced in 61 rats, resulting in a mean elevation of 5.8 mm Hg ± 1.0 (SEM) relative to the contralateral control eye. The mean duration of sustained IOP elevation (defined as >5 mm Hg relative to the control eye for at least 7 consecutive days) after a single injection was 12.8 days ± 0.9 (SEM, maximum duration 27 days). In all eyes, the visual axis remained clear from the time of injection, with minimal inflammation after injection. Retinal ganglion cell loss was determined in 21 animals (mean integral IOP, 194.5 mm Hg days ± 87.5 [SEM]) as 36.4% ± 2.4 (SEM) compared with the contralateral, untreated eye. Conclusions. The use of paramagnetic microbeads for the occlusion of the iridocorneal angle produces a sustained elevation of IOP with fewer injections and avoids the risk of visual axis occlusion. It represents a simple and effective method for the induction of experimental glaucoma.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine Optometry and Vision Sciences Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > RE Ophthalmology
Additional Information:	Supported by the National Eye Research Centre UK Grant No. SCIAD050. Confirmation received by publisher on 21 February 2014 that publisher's pdf can be self-archived 6 months after publication.
Publisher:	Association for Research in Vision and Ophthalmology
ISSN:	0146-0404
Funders:	National Eye Research Centre UK
Date of First Compliant Deposit:	30 March 2016
Last Modified:	18 May 2023 02:12
URI:	https://orca.cardiff.ac.uk/id/eprint/18362

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