Cytochrome P-450-catalysed monoterpenoid oxidation in catmint (Nepeta racemosa) and avocado (Persea americana); evidence for related enzymes with different activities

Hallahan, David, Lau, Sze-Mei C., Harder, Patricia A., Smiley, Diane W.M., Dawson, Glenn W., Pickett, John A. ORCID: https://orcid.org/0000-0002-8386-3770, Christoffersen, Rolf E. and O'Keefe, Daniel P. 1994. Cytochrome P-450-catalysed monoterpenoid oxidation in catmint (Nepeta racemosa) and avocado (Persea americana); evidence for related enzymes with different activities. Bba General Subjects 1201 (1) , pp. 94-100. 10.1016/0304-4165(94)90156-2

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Abstract

A cytochrome P-450 present in ripening avocado (Persea americana) fruit mesocarp (CYTP71A1) had previously been shown to metabolize the monoterpenoids nerol and geraniol (Hallahan et al. (1992) Plant Physiol. 98, 1290-1297). Using DNA encoding CYP71A1 as a hybridization probe, we have shown by Southern analysis that a related gene is present in the catmint, Nepeta racemosa. RNA blot analysis, together with Western analysis of catmint leaf polypeptides using avocado cyt P-450 antiserum, showed that a closely related gene is expressed in catmint leaves. Cytochrome P-450 in catmint microsomes catalysed the specific hydroxylation of nerol and geraniol at C-10, whereas avocado CYP71A1, in either avocado microsomes or heterologously expressed in yeast, catalysed 2,3- or 6,7-epoxidation of these substrates. These results suggest that orthologous genes of the CYP71 family are expressed in these two plant species, but catalyse dissimilar reactions with monoterpenoid substrates.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Chemistry
Last Modified:	10 Feb 2026 16:45
URI:	https://orca.cardiff.ac.uk/id/eprint/184638

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