The use of pluronic F-127 to study the development of the potato cyst nematode, Globodera pallida

Sasaki-Crawley, A., Curtis, R., Birkett, M., Papadoploulos, A., Blackshaw, R. and Pickett, J. ORCID: https://orcid.org/0000-0002-8386-3770 2012. The use of pluronic F-127 to study the development of the potato cyst nematode, Globodera pallida. Nematology 14 (7) , pp. 869-873. 10.1163/156854112X631944

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Abstract

This paper demonstrates a simple novel in vitro method using Pluronic F-127 aqueous solution to study the development of the potato cyst nematode, Globodera pallida, in Solanum spp. without any need for sterilisation of either the plants or the nematodes. In this study, this method was successfully applied to comparative studies on the development of G. pallida in Solanum tuberosum (potato) or S. sisymbriifolium (sticky nightshade). The protocol described here could be useful for screening transgenic plants or different plant cultivars/species for their ability to allow development not only of G. pallida but also any other plant-parasitic nematodes.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Chemistry
Publisher:	Brill Academic Publishers
ISSN:	1388-5545
Date of Acceptance:	8 February 2012
Last Modified:	11 Feb 2026 16:00
URI:	https://orca.cardiff.ac.uk/id/eprint/184688

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