Evaluation of media for susceptibility testing of Neisseria Gonorrhoeae

Davies, Leanne 2025. Evaluation of media for susceptibility testing of Neisseria Gonorrhoeae. MPhil Thesis, Cardiff University. Item availability restricted.

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Abstract

Due to its fastidious nature, susceptibility testing of N. gonorrhoeae has always been problematic. As there is no current European Committee of Antimicrobial Susceptibility Testing (EUCAST) standardised method for susceptibility testing of Neisseria gonorrhoeae, this MPhil aims to evaluate all appropriate commercially available agar plates from several manufacturers, along with evaluation of two gradient strip manufacturers, bioMérieux Etest and Liofilchem MTS to develop a Minimum Inhibitory Concentration (MIC) gradient strip method. Standard procedures of inocula preparation and primary isolation agar plates will also be investigated. All data will be analysed using the appropriate ISO standards. The outcomes of this MPhil will be to establish the most reliable agar plate and gradient strip manufacturers to perform a gradient strip MIC method, and to provide recommendations for routine diagnostic microbiology laboratories in the UK and Europe. Guidance will be published on the British Society of Antimicrobial Chemotherapy and EUCAST website. The results of this MPhil will further inform which agar plate should be used to develop a disc diffusion method in conjunction with the EUCAST Developmental Laboratory in Sweden.

Item Type:	Thesis (MPhil)
Date Type:	Completion
Status:	Unpublished
Schools:	Schools > Medicine
Date of First Compliant Deposit:	10 March 2026
Last Modified:	10 Mar 2026 09:31
URI:	https://orca.cardiff.ac.uk/id/eprint/185563

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