Expanding the scope of adenoviral vectors by utilizing novel tools for recombination and vector rescue

Fischer, Julian, Fedotova, Ariana, Bühler, Clara, Darriba, Laura, Schreiner, Sabrina and Ruzsics, Zsolt 2024. Expanding the scope of adenoviral vectors by utilizing novel tools for recombination and vector rescue. Viruses 16 (5) , 658. 10.3390/v16050658

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Abstract

Recombinant adenoviruses are widely used in clinical and laboratory applications. Despite the wide variety of available sero- and genotypes, only a fraction is utilized in vivo. As adenoviruses are a large group of viruses, displaying many different tropisms, immune epitopes, and replication characteristics, the merits of translating these natural benefits into vector applications are apparent. This translation, however, proves difficult, since while research has investigated the application of these viruses, there are no universally applicable rules in vector design for non-classical adenovirus types. In this paper, we describe a generalized workflow that allows vectorization, rescue, and cloning of all adenoviral species to enable the rapid development of new vector variants. We show this using human and simian adenoviruses, further modifying a selection of them to investigate their gene transfer potential and build potential vector candidates for future applications.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Medicine Research Institutes & Centres > Wales Cancer Research Centre (WCRC)
Publisher:	MDPI
Date of First Compliant Deposit:	10 March 2026
Date of Acceptance:	19 April 2024
Last Modified:	11 Mar 2026 14:13
URI:	https://orca.cardiff.ac.uk/id/eprint/185641

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