Abouzar, Maryam H., Poghossian, Arshak, Razavi, Arash, Besmehn, Astrid, Bijnens, Nathalie, Williams, Oliver Aneurin ORCID: https://orcid.org/0000-0002-7210-3004, Haenen, Ken, Wagner, Patrick and Schöning, Michael J. 2008. Penicillin detection with nanocrystalline-diamond field-effect sensor. Physica Status Solidi a Applications and Materials Science 205 (9) , pp. 2141-2145. 10.1002/pssa.200879713 |
Abstract
Nanocrystalline-diamond (NCD) films have been utilised for the detection of penicillin G for the first time. The developed penicillin-sensitive biosensor consists of a field-effect capacitive electrolyte-diamond-insulator-semiconductor (EDIS) structure with an immobilised enzyme layer that covers the gate region of the sensor. Undoped NCD thin films of about 100 nm thickness were grown on a p-Si-SiO2 (50 nm thermally grown SiO2) structure by microwave plasma-enhanced chemical vapour deposition. The enzyme penicillinase has been adsorptively immobilised directly onto the O-terminated NCD surface. The EDIS biosensors have been characterised in buffer solutions with different content of penicillin G by means of capacitance–voltage and constant-capacitance method. The developed penicillin biosensor possesses a low detection limit of 5 µM and a high sensitivity of 60–70 mV/decade in a wide linear range of 0.005–2.5 mM penicillin G concentration.
Item Type: | Article |
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Status: | Published |
Schools: | Physics and Astronomy |
Subjects: | Q Science > QC Physics |
Publisher: | Wiley |
ISSN: | 1862-6300 |
Last Modified: | 19 Oct 2022 08:41 |
URI: | https://orca.cardiff.ac.uk/id/eprint/18715 |
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