The promoter structure and complete sequence of the gene encoding the rabbit erythroid cell-specific 15-lipoxygenase

Oprey, J., Chester, John D. ORCID: https://orcid.org/0000-0002-7830-3840, Thiele, B. J., Janetzki, S., Prehn, S., Fleming, J. and Harrison, P. R. 1989. The promoter structure and complete sequence of the gene encoding the rabbit erythroid cell-specific 15-lipoxygenase. Gene 84 (2) , pp. 493-499. 10.1016/0378-1119(89)90526-X

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Abstract

We report the isolation and complete sequence of the gene encoding the rabbit erythroid-cell-specific 15-lipoxygenase (RBC 15-LOX), containing 14 exons spanning 8.0 kb. The transcription start point was mapped by S1 nuclease-protection experiments and comparison with the sequence of the RBC 15-LOX mRNA, as defined previously by primer extension experiments. The promoter contains a TATA-like motif, but no CCAAT motif in the canonical position, and lies within a ‘CpG-rich island’. Functional analysis of the immediate 5'-flanking DNA by transfection experiments shows that a 150 nucleotide (nt) 5'fragment linked to the chloramphenicol acetyltransferase gene acts as a functional promoter in both erythroid and nonerythroid cell lines and responds in an erythroid-specific manner to the enhancer from the Friend murine leukaemia virus long terminal repeat, whereas a 40-nt fragment is inactive. Intron 7 contains eight copies of a 54-nt repeat containing a region with homology to the simian virus 40/immunoglobulin gene enhancers.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	Gene cloning; red blood cells; CpG-rich island; gene regulation; recombinant DNA; reticulocyte; transcription
Publisher:	Elsevier
ISSN:	0378-1119
Last Modified:	19 Oct 2022 08:43
URI:	https://orca.cardiff.ac.uk/id/eprint/18809

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