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Iteratively calibrated s11 thermometry for microsecond-scale intra-pulse heating in 96-well plates

Tian, Qirui, Gao, Han ORCID: https://orcid.org/0000-0001-5890-9717, Lou, Yufei, Martin, Tracey ORCID: https://orcid.org/0000-0003-2690-4908, Jiang, Wenguo and Benedikt, Johannes ORCID: https://orcid.org/0000-0002-9583-2349 2026. Iteratively calibrated s11 thermometry for microsecond-scale intra-pulse heating in 96-well plates. Presented at: 2026 IEEE MTT-S Radio Frequency Systems and Applications Symposium (IMS RFSA), Boston, MA, USA, 7-12 June 2026. 2026 IEEE MTT-S Radio Frequency Systems and Applications Symposium (IMS RFSA). IEEE, pp. 430-433. 10.1109/imsrfsa70221.2026.11624232

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Abstract

This work is motivated by the need to separate genuine bio-electromagnetic effects from thermal artefacts in a 2.2 GHz pulsed exposure platform for 96-well cell experiments. We repurpose the system as an S11-based thermometer by performing a low-power static heating run in a 200 µ.L well, aligning S11 with a fibre-optic probe and fitting a first-order heating model. A lightweight piecewise-linear S11-temperature mapping then improves the bulk temperature accuracy from an initial 0.29 °C down to the millikelvin range, with <0.1 °C agreement over a 20 °C span. Using this calibrated mapping at MHz sampling under treatment-like conditions (1 kHz pulse trains, 100 µ.s "on" / 900 µ.s "off"), we resolve reproducible intra-pulse self-heating of ~0.2 K per burst which was completely averaged out by conventional 1–10 Hz fibre thermometry.

Item Type: Conference or Workshop Item - published (Paper)
Date Type: Publication
Status: Published
Schools: Schools > Engineering
Schools > Medicine
Publisher: IEEE
ISBN: 979-8-3315-4769-1
Date of First Compliant Deposit: 9 September 2026
Last Modified: 09 Sep 2026 10:30
URI: https://orca.cardiff.ac.uk/id/eprint/188832

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