The mapping of nucleosomes and regulatory protein binding sites on the Saccharomyces cerevisiae MFA2 gene: a high resolution approach

Teng, Yumin, Yu, Shirong and Waters, Raymond 2001. The mapping of nucleosomes and regulatory protein binding sites on the Saccharomyces cerevisiae MFA2 gene: a high resolution approach. Nucleic Acids Research 29 (13) , E64-4. 10.1093/nar/29.13.e64

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Abstract

We have developed an end-labelling approach to map the positions of nucleosomes and protein binding sites at nucleotide resolution by footprinting micrococcal nuclease (MNase)-sensitive sites. Using this approach we determined that the MFA2 gene and its upstream control regions have four positioned nucleosomes when transcription is repressed in mating type ? cells and that the nucleosomes lose their positioning when the gene became transcriptionally active in mating type a cells. We also detected MNase-hypersensitive sites in the ?2 operator region of MFA2 in ? cells but not in a cells. These probably result from the change in the local DNA conformation due to protein(s) binding in this region that governs MFA2 transcription.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Medicine
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General)
ISSN:	1362-4962
Last Modified:	26 Oct 2021 01:25
URI:	https://orca.cardiff.ac.uk/id/eprint/192

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