Riboflavin mobilization from eleocyte stores in the earthworm Dendrodrilus rubidus inhabiting aerially-contaminated Ni smelter soil

Plytycz, Barbara, Kielbasa, Edyta, Grebosz, Anna, Duchnowski, Michal and Morgan, Andrew John 2010. Riboflavin mobilization from eleocyte stores in the earthworm Dendrodrilus rubidus inhabiting aerially-contaminated Ni smelter soil. Chemosphere 81 (2) , pp. 199-205. 10.1016/j.chemosphere.2010.06.056

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Abstract

A 6-week reciprocal transfer laboratory exposure experiment was conducted with two populations of the epigeic earthworm Dendrodrilus rubidus; one population inhabited a site approx. 200 m downwind of an active Ni smelter co-contaminated with Ni and Cu (3648 and 977 μg g−1 d.w., respectively), the other inhabited uncontaminated soil. Worms transferred from unpolluted to Ni/Cu-polluted soil lost body mass (62%); they also had reduced (70%) total coelomocyte number, including autofluorescent eleocytes, and had significantly decreased (92%) riboflavin-derived fluorescence emission measured at 525 nm. Coelomocyte counts were low, and 525 nm emission was negligible in worms maintained on their native Ni/Cu soil. Earthworms and their coelomocytes were unaffected when transferred from Ni/Cu-polluted soil to unpolluted soil. In conclusion, exposing worms to stress-inducing factors, including metal pollution, alters the riboflavin status within the immune-competent cells of D. rubidus, but it requires further in vivo studies to establish whether the reduction in the fluorescence signal is predominantly due to depletion of riboflavin-containing eleocytes, or to riboflavin quenching, or to enzymatic conversion (and thus depletion) of stored riboflavin into its functional immune-potentiating flavin derivatives, FMN and FAD. The flavin budget of D. rubidus coelomocytes recovered by a reproducible extrusion procedure is a potentially useful biomarker for assessing sublethal stress in this early colonizer of disturbed soils.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QR Microbiology
Uncontrolled Keywords:	earthworms; soil metal pollution; coelomocytes; riboflavin content; autofluorescence; general stress biomarker
Publisher:	Elsevier
ISSN:	0045-6535
Last Modified:	24 Mar 2017 03:08
URI:	https://orca.cardiff.ac.uk/id/eprint/19437

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