The determination of axis lengths of dunaliella from the image analysis of TEM sections through randomly orientated cells

Roessler, J., Jones, Timothy Peter ORCID: https://orcid.org/0000-0002-4466-1260 and Berube, Kelly Ann ORCID: https://orcid.org/0000-0002-7471-7229 1996. The determination of axis lengths of dunaliella from the image analysis of TEM sections through randomly orientated cells. Biometrical Journal 38 (8) , pp. 907-920. 10.1002/bimj.4710380803

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Abstract

We present a method which improves the determination of geometric parameters of unicellular organisms with prolate ellipsoid geometry, such as Dunaliella. Approximately determined axis lenghts were obtained from transmission electron micrographs of ultrathin sections of microalgae. The density functions generated by these ‘axis lengths’ have poles which can be used to determine the short half axis length and ratio of axis lengths of the ellipsoid in order to determine the true cell volume.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Earth and Environmental Sciences
Subjects:	Q Science > QK Botany
Uncontrolled Keywords:	Stereology; Ellipsoids; Algae; Transmission Electron Microscopy; Image Analysis
Publisher:	Wiley-VCH
ISSN:	1521-4036
Last Modified:	05 Nov 2022 14:56
URI:	https://orca.cardiff.ac.uk/id/eprint/21994

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