Differential localization of GABAA receptor subunits within the substantia nigra of the human brain: an immunohistochemical study

Waldvogel, H. J., Baer, K., Gai, W.-P., Gilbert, R. T., Rees, Mark I., Mohler, H. and Faull, R. L. M. 2008. Differential localization of GABAA receptor subunits within the substantia nigra of the human brain: an immunohistochemical study. The Journal of Comparative Neurology 506 (6) , pp. 912-929. 10.1002/cne.21573

Full text not available from this repository.

Abstract

-Aminobutyric acidA (GABAA) receptors (GABAAR) are inhibitory heteropentameric chloride ion channels comprising a variety of subunits and are localized at postsynaptic sites within the central nervous system. In this study we present the first detailed immunohistochemical investigation on the regional, cellular, and subcellular localisation of 1, 2, 3, β2,3, and 2 subunits of the GABAAR in the human substantia nigra (SN). The SN comprises two major regions, the SN pars compacta (SNc) consisting of dopaminergic projection neurons, and the SN pars reticulata (SNr) consisting of GABAergic parvalbumin-positive projection neurons. The results of our single- and double-labeling studies demonstrate that in the SNr GABAA receptors contain 1, 3, β2,3, and 2 subunits and are localized in a weblike network over the cell soma, dendrites, and spines of SNr parvalbumin-positive nonpigmented neurons. By contrast, GABAARs on the SNc dopaminergic pigmented neurons contain predominantly 3 and 2 subunits; however there is GABAAR heterogeneity in the SNc, with a small subpopulation (6.5%) of pigmented SNc neurons additionally containing 1 and β2,3 GABAAR subunits. Also, in the SNr, parvalbumin-positive terminals are adjacent to GABAAR on the soma and proximal dendrites of SNr neurons, whereas linear arrangements of substance P-positive terminals are adjacent to GABAA receptors on all regions of the dendritic tree. These results show marked GABAAR subunit hetereogeneity in the SN, suggesting that GABA exerts quite different effects on pars compacta and pars reticulata neurons in the human SN via GABAA receptors of different subunit configurations.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Uncontrolled Keywords:	Human brain ; Substantia nigra ; GABAA receptor ; Immunohistochemistry
Publisher:	Wiley-Blackwell
ISSN:	0021-9967
Last Modified:	05 Jul 2013 21:45
URI:	https://orca.cardiff.ac.uk/id/eprint/22295

Citation Data

Cited 30 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item