Human primary bronchial lung cell constructs: the new respiratory models

Berube, Kelly Ann ORCID: https://orcid.org/0000-0002-7471-7229, Prytherch, Zoë Cariad ORCID: https://orcid.org/0000-0003-0690-0184, Job, Claire Alison and Hughes, Tracy G. 2010. Human primary bronchial lung cell constructs: the new respiratory models. Toxicology 278 (3) , pp. 311-318. 10.1016/j.tox.2010.04.004

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Abstract

Scientists routinely work within the three R's principles of 'Reduction, Refinement and Replacement' of animal experiments. Accordingly, viable alternatives are regularly developed, and in the specific case of the human lung, in vitro models for inhalation toxicology that mimic in vivo toxic events that may occur in the human lung, are welcomed. This is especially warranted given the new EU regulations (i.e. REACH) coming into force for the handling of chemicals and the advent of nanotoxicology. Furthermore, recent advances in human tissue-engineering has made it feasible and cost effective to construct human tissue equivalents of the respiratory epithelia, as in-house models derived from primary cells. There is an urgent need for engineered tissue equivalents of the lung given the increase in pharmaceutically valuable drugs, toxicity testing of environmental pollutants and the advent of nanotoxicology. Given the well-known problems with 2-dimensional (2-D) cell cultures as test beds, more realistic 3-D tissue constructs are required, especially for preclinical stages of cell- and tissue-based, high-throughput screening in drug discovery. The generation of high-fidelity engineered tissue constructs is based on the targeted interactions of organ-specific cells and intelligent biomimetic scaffolds which emulate the natural environment of their native extracellular matrix, in which the cells develop, differentiate and function. The proximal region of the human respiratory system is a critical zone to recapitulate for use as in vitro alternatives to in vivo inhalation toxicology. Undifferentiated normal human bronchial epithelia cells can be obtained from surgical procedures or purchased from commercial sources and used to establish 3-D, differentiated, organo-typic cell cultures for pulmonary research.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology R Medicine > R Medicine (General)
Uncontrolled Keywords:	Animal alternatives; Cell culture; Lung constructs; Pulmonary disease; Respiratory epithelium; Tissue-engineering
Additional Information:	Review
Publisher:	Elsevier
ISSN:	0300-483X
Related URLs:	http://www.ncbi.nlm.nih.gov/pubmed/20403...
Last Modified:	05 Nov 2022 15:11
URI:	https://orca.cardiff.ac.uk/id/eprint/22566

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