Culture-independent analysis of desulfovibrios in the human distal colon of healthy, colorectal cancer and polypectomized individuals

Scanlan, Pauline, Shanahan, Fergus and Marchesi, Julian Roberto ORCID: https://orcid.org/0000-0002-7994-5239 2009. Culture-independent analysis of desulfovibrios in the human distal colon of healthy, colorectal cancer and polypectomized individuals. FEMS Microbiology Ecology 69 (2) , pp. 213-221. 10.1111/j.1574-6941.2009.00709.x

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Abstract

The production of hydrogen sulphide, an end product of metabolism by the sulphate-reducing bacteria (SRB) has been cited as a potential aetiological agent in gastrointestinal disease. Quantitative PCR (Q-PCR) assays to enumerate desulfovibrios from two gastrointestinal disease groups: colorectal cancer (CRC) n=27 and polypectomized individuals (PP) n=27, and two healthy control groups, elderly (H1) n=8 and young adults (H2) n=30 was performed. Analysis of Desulfovibrio sp. diversity using the dissimilarity sulphite reductase (dsrAB) gene as a molecular marker was also undertaken. Q-PCR detected Desulfovibrio sp. in all samples and no significant difference was observed for PP, H1, H2 with gene copy numbers of Desulfovibrio sp. averaging at 106 g−1 of faeces. Significantly reduced numbers of Desulfovibrio sp. were observed for CRC (105 g−1) compared with both PP and H2 groups (P<0.05). Diversity analysis indicated that a low Desulfovibrio sp. diversity and the predominance of Desulfovibrio piger was a feature of both healthy and disease groups. In addition, a dsrAB gene sequence distantly related to a Gram-positive SRB was also recovered, highlighting the importance of cultivation-independent techniques for furthering our understanding of the diversity of the human gastrointestinal ecosystem.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	human gut; sulphate-reducing bacteria; culture-independent analysis; 16S rRNA gene; dsrAB gene; cancer
Publisher:	Wiley
ISSN:	0168-6496
Last Modified:	19 Oct 2022 10:06
URI:	https://orca.cardiff.ac.uk/id/eprint/23235

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